Mag Fura 2

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案例：

The effects of Mrs2 KD on the intracellular Mg2+ homeostasis.: The images of HeLa cells simultaneously loaded with both Mag-Fura-2 (a) and MitoFluor Red (b). (c–f) The enlarged view of the boxed area in panel a and b. (c,d) The localization of Mag-Fura-2 in the same cell shown in the panel a and b before (c) and after (d) cell membrane permeabilization by digitonin. (e) Localization of MitoFluor Red after cell membrane permeabilization by digitonin. (f) The merged image were shown. (g) The mitochondrial Mg2+ level in control and Mrs2 KD HeLa cells. Comparing the ratio of Mag-Fura-2 fluorescence excited at 340 nm to at 380 nm, indicating mitochondrial Mg2+ level, revealed a decrease in Mrs2 KD cells (n = 148 for the control and n = 105 for Mrs2 KD cells from 5 experiments). (h–j) The images of HeLa cells normally loaded with Mag-Fura-2. (h) Fluorescence image of Mrs2 KD cells labeled with emGFP. (i) The merged fluorescence images of control and emGFP- labeled (red) Mrs2 KD cells loaded with Mag-Fura-2 (green). (j) The fluorescence emission ratio image at excitation of 340 and 380 nm of HeLa cells loaded with Mag-Fura-2. Pseudo color showed the emission ratio of Mag-Fura-2 signals excited at 340 nm to 380 nm. (k) The cytosolic Mg2+ level in control and Mrs2 KD cells. In Mrs2 KD cells, cytosolic Mg2+ level is higher than that in control cells (n = 304 for the control and n = 384 Mrs2 KD cells from 5 experiments).
 

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Furaptra Tetrapotassium Salt(132319-57-4)實驗注意事項：
1.實驗前需戴好防護眼鏡，穿戴防護服和口罩，佩戴手套，避免與皮膚接觸。
2.實驗過程中如遇到有毒或者刺激性物質及有害物質產生，必要時實驗操作需要手套箱內完成以免對實驗人員造成傷害
3.實驗后產生的廢棄物需分類存儲，并交于專業生物廢氣物處理公司處理，以免造成環境污染
Experimental considerations:
1. Wear protective glasses, protective clothing and masks, gloves, and avoid contact with the skin during the experiment.
2. The waste generated after the experiment needs to be stored separately, and handed over to a professional biological waste gas treatment company to avoid environmental pollution.

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熒光圖譜

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