迪夫快速染色液(Diff-QuikStain)

一、迪夫染色液組分
Diff-Quik Fixative 迪夫固定液
Diff-Quik Solution Ⅰ 迪夫 A 液（酸性紅染液）
Diff-Quik Solution Ⅱ 迪夫 B 液（堿性藍染液）

二、產品英文介紹
Diff-Quik Stain Kit Introduction
迪夫快速染色液（Diff-Quik）是在瑞氏 - 吉姆薩染色基礎上改良而成的快速羅曼諾夫斯基染色試劑。相較于傳統瑞氏染色、瑞氏 - 吉姆薩染色，該試劑可在短時間內完成細胞分化染色，大幅縮短實驗耗時。
迪夫染色液包含三種工作液：甲醇固定液、伊紅酸性染液、亞甲藍 - 天青 A 堿性染液。細胞內嗜酸性物質與伊紅結合，呈現紅粉色；細胞核等嗜堿性結構與噻嗪類染料結合，顯藍紫色。本品染色背景干凈，細胞形態清晰，分色效果穩定，適用于實驗室大批量快速檢測。
Diff-Quik is a rapid modified Romanowsky staining reagent improved based on Wright-Giemsa stain. It can complete cell differential staining in a short time, greatly saving experimental time compared with traditional Wright staining and Wright-Giemsa staining.
The kit contains three reagents: methanol fixative, eosin-based acidic dye solution and methylene blue-azure A alkaline dye solution. Acidophilic substances in cells combine with eosin to show red-pink; basophilic structures such as cell nuclei bind to thiazine dyes to present blue-purple. The staining background is clean, cell morphology is clear, and the color separation effect is stable, which is suitable for rapid batch detection in laboratories.

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三、應用范圍
血液檢測：血涂片、骨髓涂片，白細胞分類計數
Blood examination: blood smears, bone marrow smears, leukocyte differential count
脫落細胞病理：穿刺細胞、痰液、胸腹水、陰道分泌物涂片
Cytopathology: fine needle aspiration cells, sputum, pleural and ascitic fluid, vaginal discharge smears
男科檢測：精子形態染色（WHO 精液分析標準染色方法）
Andrology test: sperm morphology staining (WHO standard for semen evaluation)
微生物與動物實驗：腹腔灌洗液、組織印片、細胞爬片快速染色
Microbiology & animal experiments: peritoneal lavage fluid, tissue touch preps, cell slide staining

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四、操作步驟
4.1 涂片自然風干
Air-dry the cell smear completely at room temperature.
4.2 固定：浸入 Diff-Quik 固定液 20 s
Immerse slide in Diff-Quik Fixative for 20 seconds for cell fixation.
4.3 染 A 液：浸泡 / 上下涮洗 5–10 s，甩掉多余染液
Stain with Solution Ⅰ for 5–10 seconds, drain excess liquid.
4.14 染 B 液：浸泡 / 上下涮洗 10–20 s
Stain with Solution Ⅱ for 10–20 seconds.
4.5 水洗：蒸餾水輕柔沖洗玻片
Gently rinse slide with distilled water to wash off unbound dye.
4.6 吸水紙吸干，鏡檢
Blot slide dry with filter paper, observe under optical microscope.

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五、染色結果
※ 細胞核、嗜堿性顆粒：藍紫色
Cell nucleus, basophil granules: blue-purple
※ 紅細胞、細胞質、基質：粉紅色 / 橘紅色
Red blood cells, cytoplasm, matrix: pink/salmon red
※ 單核、淋巴細胞胞質：淡紫紅色
Cytoplasm of monocytes and lymphocytes: light purple

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Diff-Quik Stain Kit
1. Brief Introduction
Diff-Quik stain is a rapid modified Romanowsky-type staining technique derived from Wright-Giemsa stain, officially recommended by WHO for routine cytological and hematological slide staining.
Compared with traditional Wright staining, it drastically shortens the whole staining procedure to less than 90 seconds, delivering comparable differential color discrimination with clean background and minimal precipitate, ideal for high-throughput batch staining.
The kit consists of three components: methanol-based fixative, eosin-containing acidic staining solution I, and thiazine-based alkaline staining solution II (methylene blue + Azure A).
Solution I (eosin Y, anionic dye): Stains acidophilic structures (cytoplasm, erythrocytes, cell matrix) pink-red.
Solution II (methylene blue & Azure A, cationic dyes): Stains basophilic components (cell nucleus, nucleolus, RNA-rich granules) blue-purple; metachromatic purple staining appears on lymphocyte cytoplasm and monocyte nucleiSiemens He....

2. Main Applications
Hematology: Blood smears, bone marrow smears, leukocyte differential counting
Cytopathology: Exfoliative cell smears, fine needle aspiration (FNA) specimens, vaginal discharge smears
Reproductive lab: Sperm morphology staining (WHO standard method for sperm assessment)
Histology: Frozen & paraffin section rapid screening

3. Standard Staining Protocol
Air-dry the smear naturally; immerse in Diff-Quik Fixative for 20 seconds to stabilize cellular morphology.
Dip slides up and down in Diff-Quik I for 5–10 seconds, drain excess liquid.
Dip slides up and down in Diff-Quik II for 10–20 seconds, drain excess liquid.
Rinse gently with distilled water to remove residual dye.
Blot dry with filter paper; observe under light microscope immediately. Permanent mounting with neutral balsam is optional for long-term preservation.